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anti fap apc  (R&D Systems)


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    Structured Review

    R&D Systems anti fap apc
    Anti Fap Apc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+fap+apc/Human+Fibroblast+Activation+Protein+alpha%2FFAP+Antibody/pmc13053057-87-7-9
    Average 94 stars, based on 30 article reviews
    anti fap apc - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    FACS:

    Article Title: myCAF-derived exosomal PWAR6 accelerates CRC liver metastasis via altering glutamine availability and NK cell function in the tumor microenvironment
    Article Snippet: Fresh colorectal cancer tissues were collected after surgical resection, and washed five times in phosphate-buffered saline (PBS) with 20% antibiotic–antimycotic (Gibco, USA), then minced into small pieces using ophthalmic scissors. .. Subsequently, the tissues were digested with type IV collagenase (Yeasen, China) for approximately one hour at 37 °C at 200 rpm, the digested samples were sequentially filtered through 70 μm cell strainers, the cells were then collected and centrifuged at 1500 rpm for 5 min, and the supernatant was discarded, this step was repeated 2–3 times. (FACS) using antibodies including anti–CD45- APCCy7 (BD Biosciences, no. BD- 557833), anti–EpCAM-P erCP/Cy5.5 (BioLegend, catalog no. 324214), anti–CD31- PECy7 (BioLegend, catalog no. 303118), anti–FAP- APC (primary antibody, R&D Systems, no. MAB3715), or α-SMA (BioLegend catalog no. 614851). ..

    Article Title: myCAF-derived exosomal PWAR6 accelerates CRC liver metastasis via altering glutamine availability and NK cell function in the tumor microenvironment.
    Article Snippet: Fresh colorectal cancer tissues were collected after surgical resection, and washed five times in phosphatebuffered saline (PBS) with 20% antibiotic–antimycotic (Gibco, USA), then minced into small pieces using ophthalmic scissors. .. Subsequently, the tissues were digested with type IV collagenase (Yeasen, China) for approximately one hour at 37 °C at 200 rpm, the digested samples were sequentially filtered through 70 μm cell strainers, the cells were then collected and centrifuged at 1500 rpm for 5 min, and the supernatant was discarded, this step was repeated 2–3 times. (FACS) using antibodies including anti–CD45- APCCy7 (BD Biosciences, no. BD- 557833), anti–EpCAM-P erCP/Cy5.5 (BioLegend, catalog no. 324214), anti–CD31- PECy7 (BioLegend, catalog no. 303118), anti–FAP- APC (primary antibody, R&D Systems, no. MAB3715), or α-SMA (BioLegend catalog no. 614851). ..

    Staining:

    Article Title: Deciphering the spatial landscape and plasticity of immunosuppressive fibroblasts in breast cancer.
    Article Snippet: .. To allow this characterization, cells were stained with an antibody cocktail containing anti-EpCAM−BV605 (1:50; BioLegend, # 324224), anti-CD31PECy7 (1:100, BioLegend, #303118), anti-CD45−BUV395 (1:20, BD Biosciences, #BD-563792), anti-CD235a-PerCP/Cy5.5 (1:50, Biolegend, #349109), anti-CD29-Alexa Fluor 700 (1:100, BioLegend, #303020), anti-FAP-APC (1:100, R&D Systems, #MAB3715), anti-ANTXR1-Alexa Fluor 405 (1:25, Novus Bio, #NB100-56585AF405), anti-SDC1-BUV737 (1:25, BD Biosciences, #612834), anti-LAMP5-PE (1:10, Miltenyi Biotec, #130-109-156), anti-GPC3-Alexa Fluor 549 (R&D systems, 1:20, #FAB2119T), anti-DLK1-Alexa Fluor 488 (R&D systems, 1:20, #FAB1144G), anti-CD9-BV711 (BD Biosciences, 1:100, #743050) and anti-CD74-BV786 (BD Biosciences, 1:100, #743736). ..

    Article Title: Deciphering the spatial landscape and plasticity of immunosuppressive fibroblasts in breast cancer.
    Article Snippet: .. To do so, cells were stained with an antibody mix containing anti-CD29-Alexa Fluor 700 (1:100, BioLegend, #303020), anti-FAP-APC (1:100, R&D Systems, #MAB3715), antiANTXR1-AF405 (1:25, Novus Bio, #NB100-56585AF405), anti-GPC3AF700 (1:25, R&D systems, #FAB2119N) and anti-CD74-FITC (1:50, BD Biosciences, #555540). ..

    Article Title: An Extracellular Matrix–Producing Subset of Cancer-Associated Fibroblasts Drives Chemoresistance in Breast Cancer via SRC Activation and G0S2 Upregulation
    Article Snippet: Cells were collected in DMEM high glucose (Sigma-Aldrich, #D6429), supplemented with 10% decomplemented FBS (Biosera, #FB-1001/500), 100 U/mL penicillin, and 100 μg/mL streptomycin (Sigma-Aldrich, #P4333), followed by centrifugation at 1,200 rpm for 5 minutes. .. Cells were stained with a cocktail of anti–FAP-APC (1:100, R&D Systems, #MAB3715), anti–SDC1-BUV737 (1:25, BD Biosciences, #612834), and anti–LAMP5-PE (1:10, Miltenyi Biotec, #130-109-156) for 25 minutes at room temperature in the dark. .. Then, PBS+ (= 1× PBS, supplemented with 10% FBS and 2 mmol/L EDTA, Invitrogen, #15575020) was added to each sample prior to centrifugation at 1,500 rpm for 5 minutes.

    Article Title: Deciphering the spatial landscape and plasticity of immunosuppressive fibroblasts in breast cancer
    Article Snippet: .. Cells were stained with an antibody mix containing anti-CD29-Alexa Fluor 700 (1:100, BioLegend, #303020), anti-FAP-APC (1:100, R&D Systems, #MAB3715), anti-ANTXR1-AF405 (1:25, Novus Bio, #NB100-56585AF405) and anti-LAMP5-APC (1:10, Miltenyi Biotec, #130-109-204). ..

    Article Title: Deciphering the spatial landscape and plasticity of immunosuppressive fibroblasts in breast cancer.
    Article Snippet: .. Cells were stained with an antibody mix containing anti-CD29-Alexa Fluor 700 (1:100, BioLegend, #303020), anti-FAP-APC (1:100, R&D Systems, #MAB3715), anti-ANTXR1-AF405 (1:25, Novus Bio, #NB100-56585AF405) and anti-LAMP5-APC (1:10, Miltenyi Biotec, #130-109-204). ..



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    Image Search Results


    Journal: iScience

    Article Title: Tumor-associated mesenchymal stromal cells modulate macrophage phagocytosis in stromal-rich colorectal cancer via PD-1 signaling

    doi: 10.1016/j.isci.2024.110701

    Figure Lengend Snippet:

    Article Snippet: Anti-human FAP -APC (Clone 427819) , R and D Systems , RRID: AB_2884010 ; Cat: FAB3715A.

    Techniques: Blocking Assay, Recombinant, Lysis, Saline, Flow Cytometry, Staining, Isolation, Viability Assay, CyQUANT Assay, Proliferation Assay, Software